Structure of glycolate oxidase from spinach
نویسندگان
چکیده
منابع مشابه
Preliminary crystallographic data for glycolate oxidase from spinach.
Glycolate oxidase, an enzyme that plays an important role in photorespiration in plants, has been purificant from spinach and crystallized in two different crystal forms. Form A which was obtained with tertiary butanol as precipitating agent belongs to space group I 422 with unit cell dimensions a = b = 148.1 A and c = 134.9 A. This form diffracts to high resolution and will be used for further...
متن کاملDirect expression of active spinach glycolate oxidase in Escherichia coli.
Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expres...
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The enzymatic properties and the three-dimensional structure of spinach glycolate oxidase which has the active-site Tyr129 replaced by Phe (Y129F glycolate oxidase) has been studied. The structure of the mutant is unperturbed which facilitates interpretation of the biochemical data. Y129F glycolate oxidase has an absorbance spectrum with maxima at 364 and 450 nm (epsilon max = 11400 M-1 cm-1). ...
متن کاملPeroxisomes from spinach leaves containing enzymes related to glycolate metabolism.
Microbodies, designated as peroxisomes because of their enzyme complement, have been isolated from spinach leaves. After grinding leaves in 0.5 M sucrose, the peroxisomes were removed with the broken chloroplast fraction by differential centrifugation. During sucrose density gradient centrifugation, the peroxisomes banded in about 1.9 M sucrose and were separated from mitochondria and chloropla...
متن کاملOxidative Phosphorylation and Glycolate Oxidation by Particles from Spinach Leaves.
It has recently been reported (2) that oxidative phosphorylation occurs when organic acids of the citric acid cycle are supplied as substrates to particles isolated from the cotyledons of etiolated lupine seedlings. The ratios of the number of moles of orthophosphate consumed to the number of atoms of oxygen taken up (P/0 ratios) were found to be as high as those previously reported only for an...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1985
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.82.20.6855